Use the iScript cDNA synthesis kit for first-strand cDNA synthesis for gene expression analysis using real-time qPCR. This two-tube kit is optimized to yield sensitive, unbiased representation over a broad dynamic range with minimal setup and reaction time.
2 tubes (iScript reaction mix and iScript reverse transcriptase) for faster setup
Format allows for up to 15 µl of RNA sample in a 20 µl cDNA synthesis reaction
25 x 20 µl reactions
RNaseA inhibitor protects RNA during setup and reverse transcription
iScript™ advanced cDNA synthesis kit for RT-qPCR: 2-tube format with RT capacity of input RNA up to 7.5 µg, generates cDNA in 35 min
iScript™ reverse transcription supermix for RT-qPCR: 1-tube format for total input of 1 µg–1 pg RNA, plus liquid at –20°C, eliminating freeze/thaw cycles
iScript™ Select cDNA synthesis kit: synthesize long cDNAs up to 8 kb; oligo(dT), random primer, and gene-specific primer enhancer provided in separate tubes
